| Shiranui Kyo|
|Eye Color||Light Brown|
Hired by the Choshu Domain.
Shiranui Kyo is a cocky, arrogant man who uses a gun to fight in battle. Shiranui keeps his long, wavy blue hair pulled back into a ponytail and is most often seen in all-black attire. He also tends to wear a turquoise scarf.
Harada Sanosuke seems to be his arch nemesis, as the two are always fighting one another. Shiranui works for Kazama Chikage, and appears to be loyal to him. It's unknown whether he gets along with his partner, Amagiri, but the two work together during battles.
Despite the fact that he had a rivalry with Sanosuke Harada, the two seem to be getting along better in the second season, since they call a truce to fight against the Rasetsu and Koudou. In fact, Shiranui 'sacrifices' himself so that the Shinsengumi can escape the battlefield. Later on, he informs Harada that Koudou is trying to create another war so he can feed the new Rasetsu with blood.
|Toshizo Hijikata | Chizuru Yukimura | Souji Okita | Hajime Saito | Heisuke Toudou | Sanosuke Harada | Isami Kondou | Keisuke Sannan | Shinpachi Nagakura | Genzaburou Inoue | Susumu Yamazaki | Kai Shimada | Itou Kashitarou|
|Chizuru Yukimura | Kazama Chikage | Shiranui Kyo | Kyuujyu Amagiri | Senhime | Kimigiku | Nagumo Kaoru|
|Dr. Matsumoto | Enomoto Takeaki | Ootori Keisuke|
|Others / Game-Related|
|Serizawa Kamo | Ryunosuke Ibuki | Oume | Kosuzu | Niimi Nishiki | Hirama Jyuusuke | Sasaki Aijirou | Tonouchi Yoshio|
|Okada Izou | Kirishima Kozue | Takasugi Shinsaku | Katsura Kogorou | Glover | Yamagata Aritomo | Kusaka Genzui | Itou Hirobumi | Yoshida Shiouin | Takechi Hanpeita | Kirishima Sousuke | Kiyokawa Hachirou|