| Genzaburou Inoue|
|Rank||6th Division Captain|
The team leader of the Shinsengumi sixth division, who comes from the same village as Hijikata and Kondou, and maintains a great trust and close connection with them.
Genzaburou Inoue is a kindly, middle-aged man that first appears to Chizuru when she wakes up in the Shinsengumi headquarters. He often accompanies the main Shinsengumi members during their missions, and seems to be a competent fighter. He dies after being shot and cut down by some enemies, protecting Chizuru and telling her to run. His death causes Chizuru to actually draw her sword for the first time, crying at the injustice of seeing a kind-hearted person killed in a merciless way.
His character is historically based.
|Toshizo Hijikata | Chizuru Yukimura | Souji Okita | Hajime Saito | Heisuke Toudou | Sanosuke Harada | Isami Kondou | Keisuke Sannan | Shinpachi Nagakura | Genzaburou Inoue | Susumu Yamazaki | Kai Shimada | Itou Kashitarou|
|Kazama Chikage | Shiranui Kyo | Kyuujyu Amagiri | Senhime | Kimigiku | Nagumo Kaoru|
|Dr. Matsumoto | Enomoto Takeaki | Ootori Keisuke|
|Others / Game-Related|
|Serizawa Kamo | Ryunosuke Ibuki | Oume | Kosuzu | Niimi Nishiki | Hirama Jyuusuke | Sasaki Aijirou | Tonouchi Yoshio|
|Okada Izou | Kirishima Kozue | Takasugi Shinsaku | Katsura Kogorou | Glover | Yamagata Aritomo | Kusaka Genzui | Itou Hirobumi | Yoshida Shiouin | Takechi Hanpeita | Kirishima Sousuke | Kiyokawa Hachirou|